Rapid Coomassie blue staining and destaining of polyacrylamide gels.
نویسندگان
چکیده
منابع مشابه
Removal of coomassie blue precipitates from polyacrylamide gels.
During staining of polyacrylamide gels, varying amounts of Coomassie blue may precipitate on the surface of the gels. This background obscures faint bands and is esthetically unappealing. The problem is worse when gels are stained for longer periods of time or when the stain has not been recently filtered. Rinsing a gel with methanol efficiently and quickly removes this background. Basically, a...
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Researchers in molecular biology spend a significant amount of time tending to the staining and destaining of electrophoresis gels. Here we describe a simple system, costing approximately $100 and taking approximately 1 h to assemble, that automates standard nucleic acid and protein gel staining protocols. Staining is done in a tray or, with DNA gels, in the electrophoresis chamber itself follo...
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A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-media...
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Introduction Since the first descriptions of the use of Coomassie Brilliant Blue dye to stain proteins separated electrophoretically (Fazekas De St Groth et al. 1963, Meyer and Lamberts 1965), the technique has been widely adopted for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). Further research on the colloidal properties of these dyes has led to easier, mo...
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The time required to visualize proteins using Coomassie Blue dye has been significantly reduced with the introduction of fast staining protocols based on staining with a Coomassie Blue dye solution at boiling temperatures. However, fast stainings suffer from high gel backgrounds, reducing the signal-to-noise ratio and limiting the number of detectable spots in the case of 2D SDS-PAGE. The aim o...
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ورودعنوان ژورنال:
- BioTechniques
دوره 20 3 شماره
صفحات -
تاریخ انتشار 1996